بررسی اثرات ضد میکروبی، ضد بیوفیلمی، سمیت و ترمیم زخم کرم سرد همراه با تیمول، سفوتاکسیم و سفتازیدیم در موش سوری
کد: G-1138
نویسندگان: Sajjad Jafari ℗, Mina Shirmohammadpour, Reza Akbari, Abdol‐Hakim Agh‐Atabay, Bahman Mirzaei ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Introduction: Bacterial infections from antibiotic-resistant bacteria are a major cause of death in burn patients. Our study aims to evaluate the antimicrobial, anti-biofilm, toxicity, and wound-healing effects of an ointment containing thymol, cefotaxime, and ceftazidime in cold cream using mice. Material and methods: This study was conducted in two parts: in vitro and in vivo. In the in vitro part, the antimicrobial effects of thymol, cefotaxime, and ceftazidime were tested individually and in combination on various bacterial strains. The study also investigated chemical interactions, biofilm inhibition, and cytotoxicity. In the in vivo part, the activity of the compound (F) in cold cream was examined using a mouse model of third-degree burn infection. Result: The study found that thymol/cefotaxime/ceftazidime exhibited antimicrobial activities on specific strains at varying concentrations: 8/2/2, 16/4/4, 32/8/8, 16/4/4, 64/16/16, 128/32/32 µg/ml, respectively. These compounds also showed antibacterial activity at different concentration ranges: 2-1024/1-256/2-128, 4-128/2-64/1-32, 8-256/2-128/1-128, 16-1024/2-128/2-128, 4-512/2-256/4-128, 512-1024/128-256/128-256 µg/ml on clinical strains. Analysis with FTIR revealed the presence of OH carbohydrates, proteins, and polysaccharides, CH stretching alkene group, C-O-C-polysaccharide, and C=O ester group compound in these three compounds. Moreover, compound (F) showed biofilm inhibition rates of 91.01%, 93.33%, 88.02%, 95.05%, 89.02%, and 76.02% on the mentioned strains, and its toxicity against human RBCs and Ffk cells was 18.36% and 17.26%, respectively. The burn wound area in groups one to three was larger than that of group four and the positive control group. Consequently, in the first or third group, there was better inflammation control, growth of fibroblast cells in burnt tissue, ulcer healing, and blood vessel development compared to the other groups. Discussion: The thymol/cefotaxime/ceftazidime ointment in cold cream shows strong antibacterial activity, high anti-biofilm properties, and low toxicity. It can be used in a clinical trial for infectious burn wounds.
کلمات کلیدی
antimicrobial, anti-biofilm, toxicity, wound healing, thymol, cefotaxime, ceftazidime
