اثر ضدسرطانی وکتور لنتی ویروس بیان کننده پروتئین Vpr ویروس نقص ایمنی انسانی نوع ۱ (HIV-1) در رده سلولی سرطان دهانه گردن رحم
کد: G-1157
نویسندگان: احمد طاهرپور ℗, سعید عامل جامه دار ©, سمیرا اصلی
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Background: Cervical cancer (CC) is one of the most common gynecologic malignancies, about half a million cases of cervical cancer are diagnosed worldwide. The human immunodeficiency virus type 1 (HIV-1) accessory protein, VPR, arrests the cell cycle of the G2 phase, and induced apoptosis. The viral protein R (Vpr) is packaged in the virus particle and is crucial for the replication of the HIV-1 virus in human cells. The role of VPR in the regulation of HIV-1 virus transcription has been determined, and this protein plays a role in the expression of envelope proteins, mRNA splicing, transport of the pre-integration complex to the nucleus, induction of apoptosis, activation of host genes, and inhibition of the innate immune system. this VPR-mediated apoptosis can be implicated in an efficient cancer therapy. Here, we screened new candidates for cervix cancer cell death by using recombinant pCDH-VPR recombinant plasmid. Method: Caski and Hela cervical cancer cells were transfection with pCDH-VPR recombinant plasmid, then investigated apoptosis and necrosis were undertaken using flow cytometry assay. Results: Our results indicate that exposure of Caski and Hela cells to pCDH-VPR recombinant plasmid can cause 8.4%, 11.2% and 19.5% apoptosis and 0.6%, 2.8% and 1.5% necrosis in Hela cells cervical cancer the three time periods of 24 hours, 48 hours and 72 hours, respectively. Also, lentivirus vector containing VPR gene can cause 20.5%, 39.3% and 37.7% apoptosis and 2.3%, 4.2% and 18.9% necrosis in Caski cervical cancer cell line in three time periods of 24 hours, 48 hours and 72 hours. As a result, the lentivirus vector containing the VPR gene can probably play a role in the treatment of cervical cancer. Conclusion: The purpose of this study was to investigate that pCDH-VPR recombinant plasmid are associated with anti-cancer activity against Caski and Hela cells. While the exact mechanism of the anti-cancer activity of pCDH-VPR recombinant plasmid against Hela and Caski cells remained unknown. But according to the flowcytometry assay, it seems this stategy can be indiction necrosis and apoptosis in the presence of pCDH-VPR recombinant plasmid are possible mechanisms of anti-cancer effect against hela and caski
کلمات کلیدی
Cervical cancer cell, Anti-tumour; Apoptosis, pCDH-VPR recombinant plasmid, VPR
