بررسی اثر مقایسه ای بیواکتیو تیمول/سفتازیدیم و تیمول/آمپی سیلین علیه اشریشیا کلی
کد: G-1041
نویسندگان: Mina Shirmohammadpour ℗, Sajjad Jafari, Bahman Mirzaei ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Introduction: This study aimed to compare the bioactivity of thymol/ceftazidime with thymol/ampicillin on Escherichia coli (E. coli). Material and Methods: Antimicrobial effects of thymol/ceftazidime and thymol/ampicillin were performed first individually and then combined on E. coli ATCC 25922 by MIC-MBC method. The antimicrobial effect of compounds that had synergistic effects eas performed on twenty clinical strains using the MIC-MBC method. Identification of chemical bonds, functional groups, and molecular interactions of the mentioned compounds were investigated with an FTIR device. Checkered method, time killing curve and biofilm inhibition on E. coli ATCC25922 bacteria, investigation of cytotoxicity on red blood cells (RBCs) by hemolysis method, and human skin fibroblast cells (Ffk) by MTT method were performed. thymol/ceftazidime and thymol/ampicillin (A1, and A1 compounds) had Synergistic effects. Finally, the results of the tests were compared between the two compounds. Result: The results of this study showed that the antimicrobial effects of the thymol/ceftazidime (16/8 µg/ml) were better than the thymol/ampicillin (8/2 µg/ml) in both clinical and ATCC strains. In the examination with the FTIR device, both compounds had bonds of OH carbohydrates proteins, polyphenols, C=O Amide I band, C-O-Cpolysaccharide, C-Namide III band, but one band named C=C conjugated, C≡C in both compounds showed the connection between thymol/ceftazidime and thymol/ampicillin. The biofilm inhibition effects of thymol/ceftazidime (75.51%) were better than thymol/ampicillin (62%) on E. coli ATCC25922 bacteria. The bacterial killing time curve of the thymol/ceftazidime at a lower concentration and time was better than the thymol/ampicillin. Cytotoxicity of synergistic compounds on RBCs and human Ffk cells was not different and was lower than that of Triton X-100. Discussion: The thymol/ceftazidime in this study demonstrated stronger antibacterial, anti-biofilm, and bacterial killing time effects than the thymol/ampicillin, which is not surprising given the antibiotic resistance of ceftazidime and ampicillin drugs in the treatment of disorders caused by E. coli bacteria. After more research, this combination may be prescribed to patients as a novel medication.
کلمات کلیدی
Escherichia coli, Thymol, Ceftazidime, Ampicillin, Bioactive
