مقایسه تشخیص SARS-COV-2 در سواب نازوفارنکس ، و نمونه های مدفوع کودکان بستری مبتلا به COVID-19 در بیمارستان کودکان مفید
کد: G-1046
نویسندگان: Hannan Khodaei © ℗, Leila Azimi, Maryam Rajabnejad, Fatemeh Fallah, Abdollah Karimi, Mani Shalchi Zadeh
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Introduction and Objectives: In most patients, the ability to detect SARS-CoV-2 in the upper respiratory tract ceases after 2 to 3 weeks post-symptom-onset in contrast, SARS-CoV-2 can be detected in the stool of some patients for more than 4 weeks, suggesting that stool may hold utility as an additional source for diagnosis. according to the results of studies in other countries regarding the diagnostic role of fecal PCR in patients with COVID-19, especially with gastrointestinal manifestations, we decided to perform a COVID-19 RT-PCR test on fecal samples in patients with gastrointestinal manifestations. Materials and Methods: In this cross-sectional study, 105 stool samples were obtained from children aged 1 month to 15 years with gastrointestinal symptoms suspected to be COVID-19, who were referred to Mofid Children's Hospital for RT-PCR testing of COVID-19. After total RNA extraction of stool samples and nasopharyngeal swabs sample, an RT-PCR test of COVID-19 was performed by specific primer probes, and the results were investigated. Results From 105 samples 60% was related to female and 40% of them related to male, respectively. The mean age was 2.64± 3.44 years (5 months to 14 years old). the most symptom was fever and Diarrhea. The 35% of patients had positive stool PCR. The results of GI vs. upper respiratory samples PCR show in table1. The RNA of SARS-COV2 was detected in the 18% of patient stool samples but not detected in their nasopharynx samples. Conclusion: since 42.8% of patients were confirmed as positive COVID-19 by RT-PCR on their stool samples, taking a stool sample for a COVID-19 test in children with GI manifestation is very important. also, the concentration of ACE2 receptors in the children’s digestive system is higher than in adults, and gastrointestinal manifestations become very important. if the criteria for admitting children in a clean ward is only the result of nasopharyngeal PCR, it will cause hospitalization of children with COVID-19 with digestive symptoms and negative nasopharyngeal test results in clean wards of the hospital.
کلمات کلیدی
COVID-19, Stool sample, RT-PCR test
