The Prevalence of SV40 Virus in Paraffin-Embedded Samples of Renal Cell Carcinoma and Transitional Cell Carcinoma Compared to Control Groups
کد: G-1057
نویسندگان: Sara Salimi Namin, Bahram Memar, Azadeh Soltanifar, Zahra Meshkat, Janbibi Dorazehi, Milad Bahrami ℗, Masoud Youssefi ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Introduction: The simian virus 40 (SV40) was first transmitted to human population following a contamination in polio vaccines from the monkey kidney cell cultures used to produce the vaccine in 1955-1963. In its virion structure, SV40 contains a hexamer protein called large T antigen that plays a pivotal role in viral infection and tumor formation in animals, suggesting that SV40 could be linked to cancer in humans as well. This study aimed to investigate the prevalence of SV40 virus in paraffin-embedded samples of renal cell carcinoma (RCC) and transitional cell carcinoma (TCC) compared to control groups. Methods: A total number of 140 paraffin-embedded samples were collected from the department of pathology, Imam Reza hospital, Mashhad, Iran between 2015 and 2019. Samples included 40 RCC and 40 TCC paraffin-embedded tumoral tissues as case groups and 30 non-tumoral renal and 30 non-tumoral bladder tissues as control groups. The tissues underwent deparaffinization and DNA extraction following collection. Ultimately, the Polymerase Chain Reaction (PCR) was employed to detect the viral DNA in samples. Results: The study involved 140 participants with an average age of 58.22±15.09 years, including 89 males (63.5%) and 51 females (36.4%). PCR testing revealed 1 positive and 79 negative results in the case group, while the control group had 2 positive and 58 negative results. The Chi-square test found no significant association between SV40 infection and presence of cancer (P-value=0.57). Conclusion: The current research does not establish a definitive link association between SV40 and the development of renal cell carcinoma (RCC) and transitional cell carcinoma (TCC). Considering SV40's potential tumorigenic effects and its detection in various tumors, further research is necessary to explore its role in kidney and bladder cancers.
کلمات کلیدی
SV40 Virus, Renal Cell Carcinoma, Transitional Cell Carcinoma, Polymerase Chain Reaction, Prevalence
