بررسی مقایسه ای اثرات هم افزایی تیمول با سفتازیدیم و سفوتاکسیم بر کلبسیلا پنومونیه
کد: G-1059
نویسندگان: Sajjadjafari ℗, Mina Shirmohammadpour, Arman Taran, Bahman Mirzaei ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Introduction: This study aimed to compare the antimicrobial effects of thymol/ceftazidime and thymol/cefotaxime on K. pneumoniae bacteria. Material and methods: Antimicrobial effects of thymol/ceftazidime and thymol/cefotaxime were performed first individually and then combined on K. pneumoniae ATCC 100031 by the MIC-MBC method. Therefore, the antimicrobial effects of the compounds that had a synergistic impact were performed on ten clinical strains using the MIC-MBC method. The identification of chemical bonds, functional groups, and molecular interactions of the mentioned compounds was investigated using an FTIR device. Checkered method, biofilm inhibition on K. pneumoniae ATCC 100031, cytotoxicity investigation on red blood cells (RBCs) by hemolysis and human skin fibroblast cells (Ffk) by MTT method were performed. Thymol/ceftazidime and thymol/cefotaxime had synergistic effects. Finally, the results of the tests were compared between the two compounds. Results: The results of this study showed that the antimicrobial effects of the thymol/ceftazidime (4/1 µg/ml) were better than the thymol/cefotaxime (4/2 µg/ml) in both clinical and ATCC strains. In the examination with the FTIR device, both compounds had bonds of OH carbohydrates proteins, polyphenols, C=O Amide I band, C-O-Cpolysaccharide, C-Namide III band, but one band named C=C conjugated, C≡C in both compounds showed the connection between thymol with ceftazidime and cefotaxime. The biofilm inhibition effects of thymol/ceftazidime (59.54%) were better than thymol/cefotaxime (46.36%) on K. pneumoniae ATCC 100031. The bacterial killing time curve of the thymol/ceftazidime at a lower concentration and time was better than the thymol/cefotaxime. Cytotoxicity of synergistic compounds on RBCs and human Ffk cells was not different and was lower than that of Triton X-100. Discussion: Considering the antibiotic resistance of ceftazidime and ceftazidime in the treatment of diseases caused by K. pneumoniae bacteria, the thymol/ceftazidime in this study showed better antimicrobial, anti-biofilm, and bacterial killing time effects than the thymol/cefotaxime. This combination can be used as a new drug in patients after further studies.
کلمات کلیدی
Klebsiella pneumoniae, Thymol, Ceftazidime, Cefotaxime, Synergistic
