بررسی شیوع ویروس پاپیلومای انسانی و ژنوتیپ های مختلف ویروس در جمعیت زنان و مردان نابارور
کد: G-1063
نویسندگان: Amin Abbasian ℗, Hossain Faramarzi, Niloofar Namazi, Marzieh Hosseini, Nahid Heydari Marandy, Sare Doustfateme, Nasrin Aliabadi ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
خلاصه مقاله
Background Human papillomavirus (HPV) is a prevalent sexually transmitted infection that affects both men and women, impacting the cutaneous and mucosal squamous epithelium. This study aimed to assess the prevalence of HPV in the semen of individuals with oligospermia, azoospermia, and normal sperm counts and women with HPV infection. Methods Between June 2023 and June 2024, 96 participants were included in this cross-sectional study. DNA was extracted from semen and cytology liquid brush samples, and the consensus sequences MY09/11 (18) and GP 5+/6+ (19) were used to amplify L1-region using a Real-time PCR based on SYBR-Green. Positive samples were then genotyped with the MehrViru, Iran HPV Genotyping Extra assay. Results In the oligospermic group (36 participants), 6 tested positive for HPV DNA (16.6%). In the azoospermic group, 2 out of 20 (10%) were positive, while only 3 out of 40 (7.5%) in the normal group were positive. In the cytology liquid brush, 4 out of 96 samples were positive. Statistical analysis using SPSS v.22 revealed no significant differences among the demographic data. However, independent samples t-tests indicated significant differences in sperm count (p0.05) and slow sperm motility (p0.05) in HPV-positive oligospermic samples. A total of 15 HPV genotypes were identified, with high-risk types 16 and 45, and low-risk types 6, 11, and 42 being the most common. Conclusion This study demonstrates that HPV infection can negatively impact sperm count and motility, suggesting a potential link between HPV and male fertility issues.
کلمات کلیدی
Keywords; Human papillomavirus, infertility, oligospermia, azoospermia
