خصوصیات ژن های دخیل در سیستم اکتساب آهن اسینتوباکتر بومانی مقاوم به دارو
کد: G-1031
نویسندگان: هادی حسنی ©, محمدرضا صدر ℗, نگار فروزش, مبینا جابری
زمان بندی: زمان بندی نشده!
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خلاصه مقاله:
خلاصه مقاله
This study focuses on identifying the BauA and BasD genes in Acinetobacter baumannii isolates primarily collected from Intensive Care Units (ICUs). The main goal is to design PCR primers for the simultaneous identification of these genes, with specific sequences provided. Results: The research indicates that infections caused by A. baumannii are particularly common among patients with invasive catheters and in blood samples. Identification of A. baumannii was confirmed through PCR targeting the Oxa-51 gene, revealing that 82% of multidrug-resistant (MDR) strains possess both the BauA and BasD genes. Discussion: The study highlights the critical role of iron acquisition in the pathogenesis of A. baumannii, emphasizing that iron uptake is essential for the bacterium's survival and infection within the host. It suggests the necessity for further exploration of iron acquisition mechanisms and their implications for treating A. baumannii infections, advocating for more research in this area to develop effective treatments. Materials and Methods: The analysis involved 50 MDR A. baumannii strains collected from various hospital units in Iran, including ICUs, surgical departments, and neonatal intensive care units (NICUs). Infections were linked to various sources such as blood, urine, and wounds. Confirmation of A. baumannii was achieved by amplifying the Oxa-51 gene using specific primers. Identification of the target genes was performed through conventional PCR under established experimental conditions. The findings underscore a high prevalence of the BauA and BasD genes in clinical isolates of A. baumannii, highlighting the urgent need for improved surveillance and infection control measures to curb the spread of MDR strains. The regional variation in gene frequencies suggests that tailored intervention strategies are necessary based on specific resistance mechanisms found in different healthcare settings. Further research is essential to explore the clinical implications of these findings and to develop effective strategies to address A. baumannii antibiotic resistance.
کلمات کلیدی
Acinetobacter baumannii iron acquisition pathogenesis Genes
